"/>Research area

Proteogenomics

Where the genome meets the proteome — using protein evidence to refine annotation and discover novel and variant peptides.

0
custom DB / sample
0
evidence layers
0
discovery modes
Overview

What this area is.

Proteogenomics closes the loop between DNA and protein. By searching mass spectra against sample-specific databases built from genomic variants, we confirm that predicted changes are actually translated.

This validates annotation, uncovers novel ORFs and variant peptides, and identifies candidates such as tumour neoantigens that matter for immunotherapy.

Tools & technologies

customProDBFragPipeMaxQuantGalaxy-PVEPSTAR
Gene–protein trackPeptide evidence mapped to the genome.
Variant peptidesConfirmed variant positions on a protein.
Capabilities

What we do.

Core methods we apply in proteogenomics.

Variant protein databases

Custom search spaces built from a sample's genome/transcriptome.

Peptide–spectrum matching

Confident identification against expanded databases.

Novel peptide discovery

Finding ORFs and peptides missing from references.

Variant-peptide validation

Confirming that genomic variants are translated.

Neoantigen candidates

Tumour-specific peptides for immunotherapy.

Annotation refinement

Improving gene models with protein evidence.

Workflow

From data to insight.

How a proteogenomics project flows end to end.

01

Genome/RNA

variant calls

02

Custom DB

variant proteins

03

MS search

spectra matching

04

Validate

variant peptides

05

Discover

novel ORFs

06

Apply

neoantigens · annotation

Visual analytics

Publication-grade figures.

Interactive, live-rendered visualisations used in proteogenomics.

Gene–protein trackPeptide evidence mapped to the genome.
Variant peptidesConfirmed variant positions on a protein.
Differential peptidesCondition-specific peptide changes.
Evidence networkIntegrating DNA, RNA and protein support.
Focus

Where we go deep.

Cancer neoantigens

Tumour-specific variant peptides for immunotherapy design.

Annotation refinement

Protein-level evidence correcting gene models.

Variant validation

Showing that genomic variants reach the proteome.

Insights

Questions we answer.

A few of the things people ask about proteogenomics — and our short answers. Ask CGB-AI for more.

What does proteogenomics add?

It provides protein-level proof that a genomic variant is expressed — crucial for annotation and for neoantigen discovery.

Why custom databases?

Reference protein databases miss sample-specific variants; building the search space from the genome lets those peptides be found.

Selected research

Publications in Proteogenomics.

Drawn from our full record of 173 papers, filtered to this area.

Browse all publications →

Start a proteogenomics project.

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